重复经颅磁刺激对卒中后大鼠的学习记忆功能和海马区胰岛素样生长因子-1表达的影响

关键词：卒中后认知障碍  重复经颅磁刺激  胰岛素样生长因子-1  海马区

Keywords:post-stroke cognitive impairment  repetitive transcranial magnetic stimulation  insulin-like growth factors-1  hippocampus

摘要：

      摘要 目的：研究重复经颅磁刺激(rTMS)对卒中后大鼠的学习记忆功能和海马区胰岛素样生长因子1(IGF-1)表达的影响。 方法：取成年健康雄性Wistar大鼠18只，采用线栓法建立永久性大脑中动脉闭塞大鼠模型，采用低频rTMS对实验组大鼠进行干预治疗，应用免疫荧光技术检测大鼠海马区IGF-1表达变化，应用Morris水迷宫检测大鼠空间学习记忆水平。 结果：IGF-1的表达方面，海马区的平均光密度值PMCAO组显著低于PMCAO + rTMS组和sham组(P<0.01)，PMCAO+rTMS组和sham组相比平均光密度值无显著性差异。空间学习记忆方面，在1—5天定位航行实验中，PMCAO+rTMS组和sham组大鼠的上台潜伏期显著缩短，而PMCAO组上台潜伏期的缩短趋势不如前两组显著(P<0.05)，在第6天空间探索实验中PMCAO+rTMS组和sham组大鼠的第一次找到平台位置的时间少于PMCAO组(P<0.05)，并且在60s内穿台次数多于PMCAO组(P<0.01)。 结论：低频rTMS治疗对于卒中后大鼠在学习和记忆功能的康复具有一定的临床意义，低频rTMS治疗能够在一定程度上维持大脑IGF-1的水平。

Abstract:

      Abstract Objective:To study the effect of repetitive transcranial magnetic stimulation (rTMS) learning and memory ability and the expression of IGF-1 in hippocampus region of rats after stroke. Method:Permanent middle cerebral artery occlusion (PMCAO) models were made with 18 healthy adult wistar rats. rTMS was used to intervene the PMCAO models. Immunofluorescence technique was adopted to measure the expressive change of the IGF-1 in Hippocampus. Morris water maze was used to test the learning and memory ability. Result:Compared with PMCAO group, PMCAO + rTMS and sham groups have significant lower IOD/area of IGF-1(P<0.01). There is no significant difference between PMCAO + rTMS group and sham group in IOD/area of IGF-1. In the first five days of Morris water maze test, the latency of PMCAO + rTMS and sham groups significantly reduced, whereas that of the PMCAO group was not so notably(P<0.05). In the sixth day of the water maze test, compared with PMCAO group, rats in PMCAO+rTMS and sham groups used fewer time to find the platform position in the first time (P<0.05) and crossed the platform more times within 60s (P<0.01). Conclusion:Low frequency rTMS treatment is clinically meaningful for the rehabilitation of PMCAO rats in learning and memory ability and can to some extent help to keep the level of IGF-1.