富氢水干预对耐力训练大鼠氧化-抗氧化能力的影响

关键词：富氢水  氧化应激  抗氧化  耐力训练

Keywords:hydrogen-rich water  oxidative stress  antioxidation  endurance training

摘要：

      摘要 目的：观察自由饮用富氢水8周，中等强度耐力训练大鼠氧化应激损伤指标、抗氧化指标的变化，分析富氢水对运动性氧化应激损伤的保护作用。 方法：将40只110—140g SD雄性大鼠随机分为4组：安静对照组（N=8，C组）；运动对照组（N=12，CS组）；补充富氢水组（N=8，H组）；补充富氢水运动组（N=12，HS组）。C组、CS组大鼠自由进食，饮水。H组、HS组大鼠自由进食，饮用富氢水，实验共持续8周。自第5周开始，CS组、HS组大鼠进行中等强度耐力跑台训练，训练4周。训练结束后次日，全部大鼠在24h内心尖取血，分离血清。测定血清氧化应激指标（MDA、8-OHdG、3-NT）；抗氧化指标（T-AOC、T-SOD、CAT、GSH-Px）。 结果：①大鼠血清8-OHdG、3-NT指标无组间差异；运动对照组大鼠血清MDA含量显著高于安静对照组（P＜0.05）；补充富氢水运动组大鼠血清MDA含量显著低于运动对照组（P＜0.05）。②大鼠血清T-SOD活性水平无组间差异；运动对照组大鼠血清CAT活性极显著高于安静对照组（P＜0.01）；补充富氢水运动组大鼠血清CAT活性、GSH-Px活性、T-AOC水平均显著高于运动对照组（P＜0.05）。 结论：4周中等强度耐力训练引起大鼠脂质过氧化损伤，8周自由饮用富氢水显著降低运动性氧化应激引起的脂质过氧化损伤并显著提高大鼠的抗氧化能力。

Abstract:

      Abstract Objective: To observe the effects of drinking hydrogen-rich water before and during exercising for two months on erythrocyte indexes and inflammatory indexes in rats, and to analyze the protective effect of hydrogen-rich water on sport oxidative stress injury. Method: Forty male Sprague-Dawley (110—140g) male rats were randomly divided into four groups， control group (N=8, C), exercise control group (N=12, CS group) Hydrogen-rich Water group (N=8, H group), Hydrogen-rich water exercise group (N=12, HS group). Rats in group C and group CS were free to eat and drink water. Rats in H group and HS group were fed freely and drunk hydrogen-rich water for 8 weeks. From the fifth week, rats in CS group and HS group were subjected to moderate intensity endurance treadmill training for 4 weeks. On the next day after the training, took blood from the apex of all rats within 24 hours and separated the serum. Serum oxidative stress indicators (MDA, 8-OHdG, 3-NT) were measured. Antioxidative parameters (T-AOC, T-SOD, CAT, GSH-Px) were measured. Result: ①Serum levels of 8-OHdG and 3-NT were not significantly different among the groups. Compared with the control group, the serum MDA level in the exercise control group was significantly increased（P＜0.05）. Compared with the exercise control group, MDA of Hydrogen-rich water exercise group decreased significantly（P＜0.05）. ②There was no difference among the groups in serum T-SOD activity. Compared with the control group, the serum CAT activity in the exercise control group was significantly increased (P<0.01). Compared with the exercise control group, Serum CAT activity, GSH-Px activity and T-AOC level were significantly increased in the hydrogen-rich water exercise group (P<0.05). Conclusion: Exercise stress induces oxidative damage to the lipid in rats. Long-term free drinking of hydrogen-rich water can significantly reduce oxidative damage of lipid and increase antioxidative capacity of rats.