| 吴涛,励建安,陆晓,$2,$2,孟祥博,高晶.可控性心肌缺血动物模型的制作[J].中国康复医学杂志,2006,21(12):1068~ |
| 可控性心肌缺血动物模型的制作 点此下载全文 |
| 吴涛 励建安 陆晓 $2 $2 孟祥博 高晶 |
[1]南京医科大学第一附属医院康复医学科、江苏省级机关医院内(省人民医院集团康复医学科)
,南京市珞珈路30号210024 [2]南京医科大学第一附属医院康复医学科,210029 |
| 基金项目：国家自然科学基金资助项目（30370687） |
| DOI： |
| 摘要点击次数: 2813 |
| 全文下载次数: 2881 |
| 摘要： |
| 目的：建立小型猪可控性心肌缺血的动物模型。方法：健康成年小型猪10只，开胸安置自制水囊缩窄器．建立钝缘支可控性缺血的动物模型。术后行缺血心电图试验、冠脉造影、微球技术检测缺血区心肌水囊加压前后CCBF以及靶血管支配区心肌HE染色观察。结果：制作成功的8其小型猪在水囊加压后均可出现明显的缺血性心电图表现．同时钝缘支下游显影延迟、稀疏．钝缘支近乎完全闭塞，水囊压力撤除后心电图迅速恢复正常，钝缘支远端灌注恢复到水囊加压前状态。水囊加压前后靶血管支配区域CCBF明娩减少（P〈0．05）。HE染色无心肌梗死表现。结论：采用该自制水囊缩窄器可以成功制作可控性心肌缺血动物模型。 |
| 关键词：可控性心肌缺血 动物模型 小型猪 |
| Experimental animal model of controllable myocardial ischemia Download Fulltext |
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| Dept. of Rehabilitation Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing ,210029 |
| Fund Project: |
| Abstract: |
| Objective:To establish an animal model of controllable myocardial ischemia in piglet. Method:Thoracotomy and pericardiectomy were performed in 10 piglets. A home-made hydraulic occluder was then implanted around the oblique marginal artery. Selective coronary angiography,pressure-EKG test,CCBF and pathological examination were performed after surgery to evaluate the degree of stenosis.Result:Pressure induced EKG change of ischemia could be found in all subjects. Selective coronary angiography demonstrated a significant reduction in myocardial blood flow in the oblique marginal artery after hydraulic occluder was inflated. Simultaneously,CCBF of the hazardous zone was obviously decreased(P<0.05). There was no evidence of myocardial infarction.Conclusion:The home-made hydraulic occluder is applicable to make an animal model of controllable myocardial ischemia. It may create an important approach in research of cardiac rehabilitation. |
| Keywords:controllable myocardial ischemia animal model mini swine |
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