三七促进大鼠坐骨神经损伤修复的实验研究

关键词：三七  神经再植  自噬  运动神经传导速度

Keywords:notoginseng  nerve reimplantation  autophagy  motor nerve conduction velocity

摘要：

      摘要 目的：本实验观察SD大鼠坐骨神经完全切断再植后，中药三七对大鼠自噬行为和神经修复的影响。 方法：将20只完全切断坐骨神经吻合术后大鼠随机分成中药组和空白对照组（10只/组），分别观察6周内损伤侧足趾自噬情况。于术后第6周、第10周用肌电图仪电刺激大鼠的坐骨神经远、近端，在腓肠肌肌腹部记录复合肌肉动作电位与运动神经传导速度，并与健侧对照。 结果：①三七组的大鼠自噬脚趾评分较低，与对照组比较差异有显著性意义（P<0.05）。②三七组动物损伤侧神经传导检测，复合肌肉动作电位波幅在10周时上升（P<0.05），优于对照组，差异有显著性意义。 结论：三七能够显著地减轻神经病理性疼痛，抑制大鼠的自噬行为，对周围神经再生的影响是有益的。

Abstract:

      Abstract Objective: To observe the effect of notoginseng on autophagy behavior and repairing of rat′s sciatic nerve after complete transection and anasmosis. Method: Twenty SD rats were used. The right sciatic nerves of rats were transected at the site on sciatic notch and anastomosed immediately, then randomly divided into two groups: notoginseng group and control group. The right toes autophagy behavior was observed. Electric stimulation was performed at the distal and proximal parts of sciatic nerve trunks; compound muscle action potential(CMAP) and motor nerve conduction velocity(MCV) were detected at the 6th week and 10th week after operation, and compared with the unaffected control. Result: The autophagy behavior of right toes was lighter in notoginseng group than that in control group. The amplitude of CMAP in notoginseng group was higher than that in control group. Conclusion: Notoginseng can remarkably alleviate neuropathy ache and inhibit autophagy behavior. It can also contribute to peripheral nerve regeneration after reimplantation.