电针调控细胞周期相关蛋白对局灶性脑缺血大鼠皮质细胞增殖的影响

关键词：电针  脑缺血  增殖

Keywords:electroacupuncture  cerebral ischemia  proliferation

摘要：

      摘要 目的：通过细胞周期相关蛋白探讨电针对局灶性脑缺血再灌注模型大鼠皮质细胞增殖的促进作用，阐明其治疗脑缺血的可能机制。 方法：将45只雄性SD大鼠随机分为假手术（Sham）、模型（MCAO）及电针（MCAO+EA）组，以Zea Longa法制备大鼠局灶性脑缺血再灌注模型，通过TTC和NeuN染色观察电针对脑缺血大鼠的神经保护作用，使用Brdu免疫组化法观察电针对梗死侧皮质细胞的增殖作用情况，应用Western blot和RT-PCR检测梗死侧皮质细胞周期相关蛋白的表达。 结果：电针“曲池”、“足三里”两穴可明显减轻脑缺血大鼠的脑梗死体积和神经元损伤；促进梗死侧神经细胞的增殖；并且提高细胞周期相关蛋白的表达。 结论：电针可通过调控细胞周期相关蛋白的表达，缓解脑缺血所引起的神经损伤，同时促进梗死侧皮质的神经细胞增殖，来实现对脑缺血的治疗作用。

Abstract:

      Abstract Objective: To investigate electroacupuncture (EA)-enhanced cortical cells proliferation in rats with focal cerebral ischemia-reperfusion(I/R) via cell cycle-related protein and to clarify the possible mechanism of treatment of cerebral ischemia by EA. Method: Forty-five male adult SD rats were randomly divided into sham operation control group (Sham), middle cerebral artery occlusion group (MCAO) and electroacupuncture group (MCAO+EA). The method of Zea Longa was performed to establish the focal cerebral ischemia-reperfusion (I/R) injury model. The neuroprotective effect of the EA stimulation on ischemic rats was evaluated by TTC and NeuN staining. The proliferation of cortical cells in the cerebral I/R injured rats was determined by Brdu immunohistochemical staining. The expression of cell cycle-related protein was detected by Western blot and reverse transcription PCR. Result: Electroacupuncture at the Quchi (LI11) and Zusanli (ST36) acupoints significantly reduced cerebral infarct volume, alleviated neuronal injury and enhanced cortical cells proliferation (Brdu positive) in the cerebral I/R injured rats，with the increased expression of cell cycle-related protein. Conclusion: The EA-induced increase of cell cycle-related protein may result in the promotion of cortical cells proliferation and consequently the therapeutic effect on cerebral ischemia.