基于神经血管单元稳态的电针对脑缺血再灌注损伤大鼠认知功能的影响及作用机制

关键词：脑缺血再灌注损伤  认知功能  电针  百会  神庭  神经血管单元

Keywords:cerebral ischemia-reperfusion injury  cognitive function  electroacupuncture  Baihui  Shenting  neurovascular unit

摘要：

      摘要 目的：观察电针百会、神庭穴对脑缺血再灌注模型鼠认知功能的影响并探讨其机制。 方法：健康雄性SD大鼠随机分为假手术组、模型组、电针组及非穴组，每组15只。除假手术组外，余各组均接受大脑中动脉缺血再灌注（MCAO/R）模型制备。电针组进行百会、神庭穴电针干预7d，非穴组进行百会、神庭穴向外侧平开5mm电针干预7d，模型组及假手术组仅接受同等条件抓取及固定。采用神经行为学评分和TTC染色进行模型检验，神经行为学评分评估认知功能，HE染色法观察海马区细胞形态，免疫组化观察海马区CD31、GFAP、IBa1表达，ELISA观察海马区VEGF、BDNF、IL-10表达。 结果：电针可缩短MCAO模型鼠Morris水迷宫逃避潜伏期，增加穿越平台次数，改善海马区细胞水肿、坏死及炎性细胞浸润，增加VEGF、IL-10、BDNF蛋白表达，增加CD31阳性表达，降低GFAP、IBa1阳性细胞数。 结论：电针百会、神庭穴可改善脑缺血再灌注损伤大鼠认知功能，作用机制可能与调节神经血管单元（NVU）各组分，维持NVU稳态有关。

Abstract:

      Abstract Objective： To observe the effect of electroacupuncture at Baihui and Shenting points on cognitive function in cerebral ischemia-reperfusion model rats and explore its mechanism. Method： Healthy male SD rats were randomly divided into Sham group， Model group， Electroacupuncture group， and Non-acupoint group(n=15 per group). Except for the Sham group， all other groups were prepared with a middle cerebral artery ischemia-reperfusion（MCAO/R） model. The Electroacupuncture group received 7 days of intervention with acupoints Baihui and Shenting， while the Non-acupoint group received 7 days of intervention with a 5mm outward of acupoints Baihui and Shenting. The Model group and Sham group only received the same conditions of grasping and fixation. Model testing was conducted using neurobehavioral scoring and TTC staining. Cognitive function was evaluated using neurobehavioral scoring， hippocampal cell morphology was observed using HE staining； CD31， GFAP， and IBa1 expression were observed using immunohistochemistry， and VEGF， BDNF， and IL-10 expression were observed using ELISA in the hippocampus. Result： Electroacupuncture at Baihui and Shenting acupoints can shorten the escape latency of Morris water maze in MCAO model rat， increase the number of crossing platforms， improve hippocampal cell edema， necrosis， and inflammatory cell infiltration， increase VEGF， IL-10， BDNF protein expression， increase CD31 positive expression， and reduce the number of GFAP and IBa1 positive cells. Conclusion： Electroacupuncture at Baihui and Shenting points can improve cognitive function in rats with cerebral ischemia-reperfusion injury， and the mechanism of action may be related to regulating the components of the neurovascular unit （NVU） and maintaining NVU homeostasis.