运动大鼠血浆对脊髓损伤的神经保护作用

关键词：脊髓损伤  运动血浆  神经保护  大鼠

Keywords:spinal cord injury  running plasma  neuroprotection  rat

摘要：

      摘要 目的：明确运动鼠血浆有修复脊髓损伤的作用，注入运动鼠血浆后重现运动对神经发生的有益影响，揭示运动分泌因子修复脊髓损伤的作用及机制。 方法：采用改良Allen’s法建立大鼠脊髓损伤模型，术后尾静脉分别注入运动鼠血浆或不运动鼠血浆，干预治疗28d，分别于1d、3d、7d、14d、21d、28d进行BBB评分，观察大鼠后肢运动能力的改变，HE染色评价损伤后空洞面积，Western Blot检测成熟神经元标志物NeuN的表达量。 结果：有氧运动模型构建成功，运动大鼠的体增重明显小于不运动组（n=20，P<0.001），给予运动鼠血浆后脊髓损伤大鼠后肢运动能力及灵活性明显恢复（n=6，P<0.01）；运动鼠血浆促进了伤口的愈合和毛发的生长；HE染色结果表明，与其他组相比，给予运动鼠血浆组脊髓空洞明显减少，神经元存活数量增多；Western Blot结果表明，给予运动鼠血浆组成熟神经元标志物NeuN蛋白表达显著增高（n=3，P<0.001）。 结论：运动鼠血浆可以促进脊髓损伤运动功能的恢复，其机制涉及神经元保护。

Abstract:

      Abstract Objective： To demonstrate the plasma of exercise rats has the effect of repairing spinal cord injury. After injecting the running plasma， the beneficial effects of exercise on neurogenesis are reproduced， and the mechanism of action of motor secretory factors in repairing spinal cord injury is revealed. Method： The modified Allen 's method was used to establish the model of spinal cord injury in rats. The rats with spinal cord injury were treated with exercise plasma or control plasma by tail vein injection for 28 days. Meanwhile， BBB scores were performed on 1d， 3d， 7d， 14d， 21d and 28d respectively. The changes of locomotion ability of hind limbs of rats were recorded. The cavity area after injury was observed by HE staining， and the expression level of mature nerve cell marker NeuN was detected by Western Blot. Result： The aerobic exercise model was successfully constructed. The body weight gain of rats in the exercise group was significantly lower than that in the non-exercise group （n=20，P<0.001）. The ability to move and flexibility of the hind limbs of rats with spinal cord injury were significantly improved after they were given running plasma （n=6，P<0.01）. Meanwhile， exercise plasma also can promote wound healing and hair growth. The results of HE staining showed that the syringomyelia was significantly reduced and the number of surviving neurons was increased in the exercise plasma group compared with other groups. Western Blot results showed that the expression of NeuN protein， a marker of mature neurons， was significantly increased in the group given exercise plasma （n=3，P<0.001）. Conclusion： Exercise plasma can promote the recovery of motor function after spinal cord injury， and its mechanism involves neuronal protection.