肝脂肪酶基因-514C/T多态性与糖尿病前期人群运动干预敏感性的关联研究

关键词：肝脂肪酶基因  基因多态性  糖尿病前期  运动敏感性  遗传标记

Keywords:hepatic lipase gene  gene polymorphism  prediabetes  exercise sensitivity  genetic markers

摘要：

      摘要 目的：研究肝脂肪酶基因（hepatic lipase gene，LIPC）多态性与我国北方高寒地区汉族糖尿病前期（prediabetes mellitus，PDM）人群运动干预敏感性的关联，寻找与糖代谢异常运动敏感性相关的分子标记。 方法：2022年1—9月，在哈尔滨体育学院招募32例PDM人群，进行为期12周的40%—59%VO2max健身操（3次/周，30—60min/次）结合50%—70% 1RM弹力带（2次/周，15—30min/次）运动干预。干预前后分别测试FPG、HbA1c、FINs、TC、TG、HDL-c、LDL-c，应用SNP微阵列芯片对唾液LIPC-514C/T位点进行解析，使用SPSS26.0对比不同LIPC-514C/T基因型PDM人群疗效指标的差异。 结果：①PDM人群LIPC-514C/T位点CC型占37.5%，CT/TT型占62.5%，基因频率分布符合哈温平衡（P>0.05）。②PDM人群LIPC-514 CC型HDL-c水平低于CT/TT型，具有显著性差异（P<0.05），其余指标无显著性差异（P>0.05）。③12周运动干预后PDM人群的FPG、HbA1c、TC、TG、LDL-c、HOMA-IR较干预前明显下降（P<0.05），HDL-c、FINs、HOMA-IS较干预前增加（P<0.05）。④运动干预前后CC型PDM人群的FPG、HOMA-IR、TG、HDL-c变化差值明显高于CT/TT型（P<0.05）。 结论：12周中等强度健身操结合弹力带抗阻力量训练可改善高寒地区糖尿病前期人群糖脂代谢，LIPC-514C/T位点CC型运动干预降低血糖、血脂，改善胰岛素抵抗效果优于CT/TT型，LIPC-514C/T位点可用于评价运动干预我国北方高寒地区汉族糖代谢异常人群疗效的分子标记。

Abstract:

      Abstract Objective： To investigate the association between the polymorphism of the hepatic lipase gene （LIPC）and the sensitivity to exercise intervention in the Han Chinese population with prediabetes mellitus （PDM） in the cold northern region of China， and to identify molecular markers related to exercise sensitivity in glucose metabolism disorders. Method： From January to September 2022， 32 PDM patients were recruited in Harbin sports university，and 40%—59% VO2max aerobic exercise（3 times/week， 30—60min/time） combined with 50%—70% 1RM elastic band training（2 times/week， 15—30 min/time）was performed for 12 weeks. FPG， HbA1c， FINs， TC， TG， HDL-c and LDL-c were measured before and after the intervention， SNP microarray chip was used to analyze the LIPC-514C/T locus in saliva， and SPSS26.0 was used to compare the differences in efficacy indicators among PDM patients with different LIPC-514C/T genotypes. Result： ①The CC type of LIPC-514C/T locus accounted for 37.5%， and CT/TT accounted for 62.5% in PDM patients. The gene frequency distribution was consistent with Hardy-Weinberg equilibrium （P>0.05）. ②The HDL-c level of LIPC-514 CC type in PDM patients was lower than those in CT/TT type， with a significant difference（P<0.05）， while there was no significant difference in other indicators （P>0.05）. ③After 12-week exercise intervention， the levels of FPG， HbA1c， TC， TG， LDL-c and HOMA-IR were significantly lower（P<0.05）， and HDL-c， FINs and HOMA-IS were significantly higher than those before intervention（P<0.05）. ④The change in FPG， HOMA-IR， TG and HDL-c in CC type PDM patients was significantly higher than those in CT/TT genotype after intervention（P<0.05）. Conclusion： A 12-week moderate-intensity aerobic exercise combined elastic band resistance strength training can improve the glucose and lipid metabolism in PDM in cold region. The effect of exercise intervention in reducing blood compared to the CT/TT genotype of the LIPC-514C/T locus, which can be used as a molecular marker to evaluate the efficacy of exercise intervention in the Han Chinese population with glucose metabolism disorders in cold region of northern China.