右-苯丙胺对脑缺血大鼠神经丝蛋白和微管相关蛋白表达的影响

关键词：脑缺血 微管相关蛋白 神经丝蛋白 右-苯丙胺

Keywords:cerebral ischemia  D-amphetamine  neurofilament protein  microtuhule-associated protein2

摘要：

      研究右-苯丙胺对大鼠大脑中动脉闭塞MCAO后神经丝蛋白（NF）、微管相关蛋白（MAP2）表达的影响，并在分子水平上探讨其加速神经康复的机制。方法：应用Koizumi线栓法建立单侧MCAO模型，用免疫组织化学方法及RT-PCR检测缺血周围区MAP2、NF表达的变化。结果：在MCAO 1周末MAP2、NF的表达明显低于假手术组，以后逐渐增高，在第6周末其表达高于假手术组，模型＋右-苯丙胺组在各时间点其表达均明显高于模型组。结论：右-苯丙胺可促进脑缺血后动物的MAP2、NF的表达，提高神经可塑性，可能为其加速神经康复的分子机制之一。

Abstract:

      Objective:To study the effects of D-amphetamine on the expression of microtubule-associated protein2 (MAP2) and neurofilament protein(NF) after middle cerebral artery occlusion(MCAO),and explore the molecular mechanism of D-amphetamine in accelerating rehabilitation in rats with acute cerebral infarction.Method:The unilateral MCAO models were induced by using Koizumi's method. The changes in the expression of MAP2?NF around ischemia area was observed by reverse transcriptase-polymerase chain reaction(RT-PCR) and immunohistochemical techniques.Result:Compared with the sham-operated group,the expression of MAP2 and NF evidently decreased at 1 week,then increased gradually. Both the expression of MAP2 and NF proteins demonstrated statistically significant increased in D-amphetamine treatment group compared with natural recovery group all time points. Same results appeared in RT-PCR product.Conclusion:D-amphetamine effectively promotes the expression of MAP2 and NF around ischemia area, which indicates that neural plasticity is molecular mechanism of D-amphetamine in accelerating rehabilitation in rats with acute cerebral infarction.