吴涛,励建安,陆晓,$2,$2,孟祥博,高晶.可控性心肌缺血动物模型的制作[J].中国康复医学杂志,2006,21(12):1068~ |
可控性心肌缺血动物模型的制作 点此下载全文 |
吴涛 励建安 陆晓 $2 $2 孟祥博 高晶 |
[1]南京医科大学第一附属医院康复医学科、江苏省级机关医院内(省人民医院集团康复医学科) ,南京市珞珈路30号210024 [2]南京医科大学第一附属医院康复医学科,210029 |
基金项目:国家自然科学基金资助项目(30370687) |
DOI: |
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摘要: |
目的:建立小型猪可控性心肌缺血的动物模型。方法:健康成年小型猪10只,开胸安置自制水囊缩窄器.建立钝缘支可控性缺血的动物模型。术后行缺血心电图试验、冠脉造影、微球技术检测缺血区心肌水囊加压前后CCBF以及靶血管支配区心肌HE染色观察。结果:制作成功的8其小型猪在水囊加压后均可出现明显的缺血性心电图表现.同时钝缘支下游显影延迟、稀疏.钝缘支近乎完全闭塞,水囊压力撤除后心电图迅速恢复正常,钝缘支远端灌注恢复到水囊加压前状态。水囊加压前后靶血管支配区域CCBF明娩减少(P〈0.05)。HE染色无心肌梗死表现。结论:采用该自制水囊缩窄器可以成功制作可控性心肌缺血动物模型。 |
关键词:可控性心肌缺血 动物模型 小型猪 |
Experimental animal model of controllable myocardial ischemia Download Fulltext |
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Dept. of Rehabilitation Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing ,210029 |
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Abstract: |
Objective:To establish an animal model of controllable myocardial ischemia in piglet. Method:Thoracotomy and pericardiectomy were performed in 10 piglets. A home-made hydraulic occluder was then implanted around the oblique marginal artery. Selective coronary angiography,pressure-EKG test,CCBF and pathological examination were performed after surgery to evaluate the degree of stenosis.Result:Pressure induced EKG change of ischemia could be found in all subjects. Selective coronary angiography demonstrated a significant reduction in myocardial blood flow in the oblique marginal artery after hydraulic occluder was inflated. Simultaneously,CCBF of the hazardous zone was obviously decreased(P<0.05). There was no evidence of myocardial infarction.Conclusion:The home-made hydraulic occluder is applicable to make an animal model of controllable myocardial ischemia. It may create an important approach in research of cardiac rehabilitation. |
Keywords:controllable myocardial ischemia animal model mini swine |
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