李红玲,曹慧芳,田 苗,张 丽,李春岩.运动训练对大鼠试验性脑出血后血肿周围组织细胞凋亡的影响[J].中国康复医学杂志,2008,23(2):103~106 |
运动训练对大鼠试验性脑出血后血肿周围组织细胞凋亡的影响 点此下载全文 |
李红玲 曹慧芳 田 苗 张 丽 李春岩 |
河北医科大学第二医院康复科,石家庄市和平西路215号,050000 |
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摘要: |
目的: 观察运动训练对血肿周围组织细胞凋亡的影响。方法: 120只SD大鼠被随机分为3组,实验组(40只,出血并运动)、对照组(40只,出血不运动)和假手术组(40只,无出血,不运动),前两组又分为术后24h,7d,14d,21d,28d,5个时相点,每个时相点3只用于TUNEL,5只用于流式细胞仪检测。 结果: TUNEL阳性细胞数在脑出血(ICH)24h时实验组和对照组无区别,但与假手术组相比差异有非常显著性(P<0.01),凋亡于ICH术后7—14d一度下降,21d后回升,28d又下降,但实验组表达明显低于对照组(P<0.05)。凋亡不仅存在于血肿周围组织和皮质,海马区也可见明显凋亡现象。流式细胞分析:不同时间细胞凋亡率的变化趋势与TUNEL 阳性细胞数一致,而且实验组凋亡率明显低于对照组,差异有显著意义(P<0.05), 但双参数分析较单参数灵敏性更高,差异有非常显著意义(P<0.01)。结论:运动训练可以通过抑制脑出血后血肿周围的神经细胞凋亡,从而改善神经功能。 |
关键词:运动训练 脑出血 神经细胞凋亡 流式细胞分析 大鼠 |
The physical exercises effect on apoptosis of neuronal cells surrounding intracerebral hemorrhage in rats Download Fulltext |
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Dept. of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 |
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Abstract: |
Objective: To investigate the effects of cage-running exercises on apoptosis of neuronal cells surrounding the hematoma in ICH rats. Method: A total of 120 male SD rats were randomly divided into three groups, trial group (ICH with EX n=40), control group( ICH with no EX n=40) and sham operated group (no ICH, no EX n=40). The rats brains were harvested at the 24th h,7th d,14th d,21st d,28th d after ICH. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-bioti in situ nick end-labeling(TUNEL) was used to detect deoxyribonucleic acid(DNA) fragmentation. Flow cytometry assay(FCA) was quantified for DNA. Result: ①TUNEL-positive cells appeared in the periphery of hematoma and hippocampus. The number of TUNEL-positive cells was nearly zero in the sham-operated group, and this number increased markedly in control group from the 14th d to 28th d after ICH, but it reduced in trial group at the same time. Even though, the number of TUNEL-positive cells in two groups once decreased from the 7th—14th d, but the number of TUNEL-positive cells in trial group was less than that in control group. There was a significant difference between two groups(P<0.05). ②The apoptotic regularity of flow cytometry assay was similar to that of TUNEL-positive cell. But the apoptotic rate of neuronal cells in Annexin Ⅴ-P was more higher than that in PI. Conclusion: Exercises training (cage-running) can inhibit the number of apoptotic cells. |
Keywords:exercises training intracerebral hemorrhage apoptosis of neuronal cells flow cytometry assay rats |
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