电针刺激对大鼠脑缺血再灌注后PI3-K/Akt通路的影响*

关键词：电针  脑缺血再灌注  凋亡  PI3-K/Akt  

Keywords:electroacupuncture  cerebral ischemia reperfusion  apoptosis  PI3-K/Akt

摘要：

      目的：研究电针刺激百会和大椎两穴对脑缺血再灌注大鼠脑神经细胞凋亡的影响, 并通过对皮质及纹状体缺血周围区PI3-K和Akt的检测, 进一步探讨电针刺激减少神经细胞凋亡的分子机制。方法：雄性SD 大鼠, 采用Zea Longa线栓法制备大鼠大脑中动脉闭塞(MCAO)模型, 缺血3h后再灌注。分为假手术组、模型组、电针组，分别于手术前12h、手术前30min、手术后每隔12h针刺百会、大椎穴1min，再通以电针治疗仪予疏密波治疗15min，直到48h后最后一批大鼠被处死；术后分第12、24、48小时三个时间点

Abstract:

      Objective: To study the effects of electroacupuncture(EA) on phosphatidylinositol 3-kinase(PI3-K), serine/threonine kinase(Akt) path and apoptosis in rats brains following local cerebral ischemia/reperfusion. Method: Male adult SD rats were randomly divided into three groups: the sham-operated group, the model group and the EA group. The model of middle cerebral artery occlusion(MCAO) was established by thread ligation method. Neurological system symptoms were evaluated at the 12thh, 24thh, 48thh after ischemia/reperfusion. Then the rats were sacrificed at the 12thh, 24thh, and 48thh after reperfusion, and rats brains were taken for histopathological, TUNEL and immunohistochemistry examinations. Positive reacted cells of apoptosis, PI3-K and Akt were counted under light microscope at different time points after ischemia/reperfusion. Result: EA could significantly alleviate the damage of neurological system; histopathological examination showed that the cerebral ischemic damage in the EA group was significantly milder than that in model group. As compared with sham-operated group and model group, positive reacted cell amounts of apoptosis were greatly declined in EA group（P<0.05）. Positive immune reacted cell amounts of PI3-K and Akt were greatly enhanced in EA group as compared with model group and sham-operated group. Conclusion: The protective effects of EA therapy against cerebral ischemia/reperfusion injury might be associated with improving the positive expressions of PI3-K and Akt path.