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刘晓刚,邓宇斌,蔡 辉,何杰民,沈慧勇.诱发电位在GDNF与MSCs联合移植法治疗猴脊髓损伤评价中的应用*[J].中国康复医学杂志,2009,24(6):509~511
诱发电位在GDNF与MSCs联合移植法治疗猴脊髓损伤评价中的应用*    点此下载全文
刘晓刚  邓宇斌  蔡 辉  何杰民  沈慧勇
北京市垂杨柳医院病理科,北京,100022
基金项目:国家自然科学基金(30271327)
DOI:
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摘要:
      目的:应用诱发电位技术评价控释胶质细胞源性神经营养因子(GDNF)与骨髓间充质干细胞(MSCs)源性神经元样细胞联合移植对猴脊髓损伤的治疗效果是否优于单纯细胞移植。方法:应用改良Allen′s法制作猴脊髓损伤模型,实验组(4只)给予控释GDNF与MSCs源性神经元样细胞移植,对照组(4只)给予单纯MSCs源性神经元样细胞移植。进行运动诱发电位(MEP)和皮质体感诱发电位(CSEP)检测,比较两组间治疗4—5个月后诱发电位的差异。结果:联合移植组MEP潜伏期较单纯细胞移植组缩短(P<0.05)、波幅较单纯细
关键词:诱发电位  脊髓损伤  间充质干细胞  神经营养因子  猕猴
Evoked potential in evaluating the effect of combined transplantation of GDNF and MSCs on spinal cord injury of mucaca multta    Download Fulltext
Department of Pathology, Chui Yangliu Hospital of Beijing, 100022
Fund Project:
Abstract:
      Objective: To evaluate the effect of combined transplanfation of controlled-release biomaterial of glial cell line-derived neutrophic factor(GDNF) and mescenchymal stem cells(MSCs) induced prophase neuron-like cells on spinal cord injury repairing in mucaca multta with evoked potential technology. Method:Modified Allen′s method was introduced to make spinal cord injury model. In one group mucaca multtas(n=4) were transplanted with controlled-release biomaterial of GDNF and MSGs induced prophase neuron-like cells and in another group mucca multta(n=4) were transplanted with only MSCs induced Prophase neuron like cells. Motor evoked potential(MEP) and cortical somatosensory evoked potential(CSEP) were detected at the time of pre-transplantation and 4—5 months after transplantation. Result:MEP and CSEP disappeared completely before transplantation and recovered partially 4—5 months after transplantation. Only the difference of MEP between two groups had statistical significance. Conclusion:Combined transplantation of GDNF and MSCs for spinal cord injury presents much more promising prospect than only cells transplantation.Evoked potential technology is a meaninful method to evaluate the effect for spinal cord injury.
Keywords:evoked potential  spinal cord injury  mesenchymal stem cells  neurotrophic factor  mucaca multta
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