脉冲磁场对脑缺血再灌注大鼠胰岛素生长因子表达的影响*

关键词：脉冲磁场  脑缺血  再灌注损伤  胰岛素生长因子

Keywords:pulsed magnetic field  brain ischemia  reperfusion injury  insulin-like growth factor-1

摘要：

      目的：观察脉冲磁场对脑缺血再灌注大鼠胰岛素生长因子(IGF-1)表达的影响。方法：SD大鼠24只,随机分成假手术组、模型组和脉冲磁场组,每组8只。采用线栓法制备大鼠局灶性脑缺血模型, 假手术组仅做右侧颈外动脉和颈总动脉结扎，不做右侧大脑中动脉线栓栓塞。脉冲磁场组在造模结束后2h给脉冲磁场处理，磁场强度为0—0.01T，频率为50Hz，20min/次，1次/d，7d后处死大鼠,断头取脑,采用免疫组化方法检测IGF-1的表达。结果：模型组与假手术组相比,IGF-1的表达增多；脉冲磁场组与模型组相比,IGF-1的表达增多(P<0.05)。结论：脉冲磁场能促进脑缺血再灌注大鼠IGF-1的表达。

Abstract:

      Objective:To examine the effect of pulsed magnetic field in inducing insulin-like growth factor-1(IGF-1) expression in focal cerebral ischemia reperfusion injured model. Method:Twenty-four SD rats were randomized into 3 groups, namely, sham-operation group, model group and pulsed magnetic field group with 8 in each group. Middle cerebral artery occlusion(MCAO) was employed to establish focal cerebral ischemia prefusion model in rats of model group and pulsed magnetic field group. In sham-operation group rats′ middle cerebral artery were only ligated without occlusion. The treatment of pulse magnetic field was at 50Hz, 0.01T, 20min, once daily, for 7d. The expression of IGF-1 in ischemic brain tissue was detected with immunohistochemical staining. Result:The expression of IGF-1 in ischemic brain tissue was the highest in pulsed magnetic field group. Conclusion:Pulsed magnetic field might improve the expression of IGF-1 in cerebral ischemia reperfusion injured rats.