不同强度运动对大鼠自由基代谢影响的研究

关键词：运动  自由基  代谢  血红蛋白  丙二醛  总抗氧化能力

Keywords:exercises  free radicals  metabolism  hemoglobin  malonaldehyde  total antioxidant cupacity

摘要：

      目的：研究不同强度运动对大鼠血清、骨骼肌、心肌、肝脏中自由基代谢的变化。方法：18只雄性Wistar大鼠随机分为安静对照组（CG）、适度运动组（MG）和运动性低血色素组（SG），每组6只。5周递增负荷跑台运动后测定血清、腓肠肌、心肌、肝脏丙二醛（MDA）、总抗氧化能力（T-AOC）。结果：经5周递增负荷跑台运动后，MG组T-AOC活性在血清、腓肠肌、肝脏中显著高于CG组（P<0.01）；SG大鼠各组织中MDA含量明显高于CG和MG（P<0.01），T-AOC活性（除心肌外）明显低于MG（P<0.01）。结论：①适度运动可提高大鼠各组织总抗氧化能力，增强机体清除自由基的能力。②长时间大强度递增负荷跑台运动导致的总抗氧化能力下降，脂质过氧化反应增强是引起运动性低血色素的重要原因之一。

Abstract:

      Objective: To investigate the metabolism of free radicals in rats after different intensity exercises training. Method: Eighteen male Wistar rats were randomly divided into three groups: control group (CG), moderate exercises group (MG) and exercises-hypochrosis group (SG). The serum, heart, gastrocnemius and liver were sampled after 5-week different intensity treadmill exercises. The Hb,malonaldehyde(MDA) and total-antioxidant capacity(T-AOC) were measured. Result: The T-AOC of serum, gastrocnemius, liver in MG increased significantly than that in CG and SG(P<0.01). The MDA of serum, heart, gastrocnemius and liver in SG increased significantly compared with that in CG and MG(P<0.01). While the T-AOC in SG decreased significantly compared with that in MG (P<0.01). Conclusion: ①The total antioxidant capacity and free radical scavenging ability of the body enhanced after moderate exercises. ②The decreased total antioxidant capacity and the heightened free radical metabolism was one of the important causes of exercises-induced hypochrosis.