李俊岑,丁培培,刘姿辰,张 剑,梁 楠,张 晓.神经前体细胞巢蛋白在大鼠脊髓损伤后的表达[J].中国康复医学杂志,2010,25(9):833~837 |
神经前体细胞巢蛋白在大鼠脊髓损伤后的表达 点此下载全文 |
李俊岑 丁培培 刘姿辰 张 剑 梁 楠 张 晓 |
成都医学院, 610081 |
基金项目:四川省科技厅课题(06J13-023);成都医学院创新实验项目(2009154) |
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摘要
目的:探讨成年大鼠脊髓损伤后损伤(SCI)区局部巢蛋白(nestin)的表达及意义。
方法:应用Allen法建立大鼠SCI模型, 行为学评分采用BBB 评分,用病理学和免疫组织化学方法检测脊髓在不同时段的病理改变和nestin的表达变化。
结果:伤后第1天, 脊髓实质灶状出血,小血管栓塞,部分神经细胞细胞核碎裂,见损伤区附近、软脊髓膜下的白质和脊髓中央管区有nestin表达,BBB评分低,随后增加,1—2周恢复幅度加大。第3天后损伤灶出现大量胶质细胞,损伤组织液化。第5天后液化灶逐渐扩大,出血减少,阳性神经元和阳性反应的平均积分光密度值达到高峰(P<0.05)。第7天后神经细胞退行性变更为严重,部分神经细胞崩解仅留其轮廓,胶质细胞增生明显。2周后出血已基本吸收,以损伤处为中心,囊腔开始形成, nestin表达明显下调(P<0.05)。
结论:脊髓损伤可诱导损伤区周围短暂的nestin阳性表达, nestin可能在脊髓损伤后的再生与修复中起重要作用。 |
关键词:脊髓损伤 巢蛋白 Allen撞击器 Basso, Beattie, Bresnahan运动功能评分 |
Expression of nestin of neural precursor cell after spinal cord injury in rats Download Fulltext |
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Chengdu Medical College Experimental Technology Center, Chengdu Sichuan Province, PRC, 610081 |
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Abstract: |
Abstract
Objective:To study the expression and significance of nestin in the areas adjacent to the injured site after spinal cord injury(SCI) in rats.
Method:Each rat received contusion on its spinal cord at T9 with modified Allen′s impactor to establish SCI model. The behavioral evaluation was performed with Basso, Beattie & Bresnahan locomotor rating scale (BBB scale).The pathological study included hematoxylin and eosin (HE) staining, and immunohistology staining for nestin.
Result: The spinal cord lesion as bleeding, small vessel thrombosis, some nerve cell karyorrhexis in myeloid tissue could be seen at the 1st d after injury. The expression of nestin could be seen near the damage zone, spinal cord white matter under spinal pia-mater and the central canal of spinal cord.BBB score lowered at the 1st d after injury, and the recovery range increased gradually in 1—2 weeks.There were a lot of glial cells proliferation, tissue liquefaction in damaged zone at the 3rd d after injury. Liquefaction area of damage areas expanded gradually, bleeding reduction, and the average integral optical density of nestin-immunoreactive neurons reached the peak (P<0.05) at the 5th d after injury. Nerve cells occured serious degeneration, some nerve cells appeared necrosis, with marked hyperplasia of glial cells at the 7th d after injury. Two weeks later the bleeding absorbed basically, cysts began to form around the damage center and the nestin expression reduced significantly(P<0.05).
Conclusion:SCI can induce the transient expression of nestin in the areas adjacent to injured site, which may play an important role in the repair and restoration of spinal cord. |
Keywords:spinal cord injury nestin Allen′s impactor Basso, Beattie & Bresnahan locomotor rating scale |
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