12周跑台训练对非酒精性脂肪肝小鼠肝脏肝细胞癌下调的线粒体转运蛋白表达的改变及其对线粒体功能的影响

关键词：非酒精性脂肪肝  耐力训练  肝细胞癌下调的线粒体转运蛋白  线粒体功能

Keywords:non-alcoholic fatty liver disease  endurance training  HCC-down-regulated mitochondrial carrier protein  mitochondrial function

摘要：

      摘要 目的：研究12周跑台训练后非酒精性脂肪肝（NAFLD）小鼠肝脏肝细胞癌下调的线粒体转运蛋白（HDMCP）的变化及其对肝脏线粒体功能的影响。 方法：雄性8周龄ApoE-/-小鼠48只，随机分为4组，对照组（C，n＝12），14周西方膳食组（模型组，M，n＝12），继续西方膳食＋12周跑台运动组（ME，n＝12）；西方膳食26周组（MM，n＝12）。跑台训练12周，坡度为0°，跑速13m/min，60min/次，每周5次。测定肝脏HDMCPmRNA的表达，线粒体活性氧及H2O2生成，线粒体ATP合成活力，肝脏ATP含量，线粒体态3呼吸、态4呼吸及呼吸控制比（RCR）。 结果：耐力训练组（0.53±0.09）肝脏HDMCPmRNA表达显著低于C组（0.72±0.10）、M组（1.24±0.16）、MM组（1.32±0.17）；耐力训练组肝脏线粒体ATP合成活力（11.23±2.53）及肝脏组织ATP含量（10.57±2.12）均显著高于MM组（7.36±1.22，8.32±1.41）；耐力训练组肝脏线粒体ROS（42.51±4.12）及H2O2生成（18.33±3.10）均显著低于MM组（68.94±5.99，24.31±3.56）；耐力训练组线粒体态3呼吸（12.21±1.32）与RCR（2.78±0.89）均显著高于MM组（10.76±1.11，1.75±0.69），而态4呼吸（4.40±1.32）显著低于MM组（6.14±1.45）。 结论：12周跑台训练可以显著降低肝脏HDMCP表达，可能进而减少线粒体质子漏，增强线粒体ATP合成，改善线粒体功能。

Abstract:

      Abstract Objective: To investigate the change of 12-week treadmill training on the HDMCP expression of liver tissue and its effect on mitochondrial function in no-alcholic fatty liver disease (NAFLD)mice. Method: Forty-eight male ApoE-/- mice were divided into four groups randomly：①14-week normal diet group(C group, n=12); ②14-week western diet model group(M group, n=12); ③western diet plus 12-week treadmill training group(ME group, n=12);　④26-week western diet (MM，n＝12). Mice were trained on a treadmill for 12 weeks，60 minutes per day，5 days per week, 0°grade (at 13m/min). Every period ended, mice were sacrificed and liver was saved for measuring the expression of HDMCP mRNA, and the change of activity of ATP synthetase, and variations of state 3 respiration,state4 respiration and RCR，mitochondrial ROS production and content of ATP in liver tissue. Result: The expression of HDMCP in ME group(0.53±0.09) was significantly lower than that in C group(0.72±0.10), M group (1.24±0.16) and MM group(1.32±0.17). The activity of ATP synthetase(11.23±2.53) and content of ATP in liver tissue(10.57±2.12) in ME group were significantly higher than those in MM group(7.36±1.22，8.32±1.41). The ROS (42.51±4.12) and H2O2(18.33±3.10) production of mitochondria in ME group were significantly lower than those in MM group(68.94±5.99，24.31±3.56). The state3 respiration(12.21±1.32) and RCR(2.78±0.89) in ME group were significantly higher than those in MM group(10.76±1.11，1.75±0.69), however, state4 respiration (4.40±1.32) was lower than that in MM group(6.14±1.45). Conclusion：Twelve training significantly decreases HDMCP expression, which maybe decrease mitochondrial proton leak, enhance the synthesis of ATP of mitochondria，and ameliorate mitochondrial function in NAFLD mice.