Effects of Electro-acupuncture Preconditioning on Focal Cerebral Blood Flow in Artery and Vein of Rats with Middle Cerebral Artery Occlusion

Keywords:electro-acupuncture preconditioning  middle cerebral artery occlusion  cerebral blood flow

摘要：

      

Abstract:

      Abstract Objective：To investigate the effects of electro-acupuncture preconditioning on focal cerebral blood flow (CBF) in artery and vein of rats with middle cerebral artery occlusion(MCAO), and to provide experimental evidences for primary prevention of ischemic stroke. Method：Eighteen male Sprague-Dawley rats (two months old) were divided into 3 groups: electro-acupuncture preconditioning group (EA group), ischemia group (Is group) and Dazhui and Baihui sham group (six rats per group). The rats in the EA group were given electro-acupuncture preconditioning at Dazhui and Baihui with 2/15 density wave current for 30 minutes per day, 5 days consecutively. After preconditioning, enhanced laser speckle contrast analysis was implemented to get false color images before making middle cerebral ischemia occlusion model. Then getting false color images two hours during ischemia by laser speckle contrast analysis again. Result：①Relative CBF in focal cortical artery: There were significant differences at every time point in EA group and Is group (P<0.01, P<0.05) comparing with that before occlusion respectively. Compared with that before ischemia, there was significant difference at 120 min after ischemia in sham group (P<0.05); compared with that at 30 min after occlusion, there was significant difference at every time point in EA group from the time point 60 min after occlusion; there was significant difference at every time point in EA group and Is group (P<0.05) comparing with sham group; Compared with sham group, there was significant difference at 30 min and 60 min after ischemia in EA group (P<0.05). ② Relative CBF in focal cortical vein: There was significant difference at every time point in sham group comparing with that before occlusion (P<0.05); compared with that before occlusion, there was significant difference from 45 min after occlusion in EA group (P<0.05); compared with that before occlusion, there was significant difference from 105 min after occlusion in sham group (P<0.05); there was significant difference at 120 min after occlusion comparing with 60 min after occlusion in EA group; compared with sham group, there was significant difference at every time point in EA group and Is group (except 30 min after occlusion) (P<0.05), respectively; compared with Is group, there was significant difference at 30, 45, 60 min after occlusion in EA group (P<0.05). ③ The infarct brain volume detected 24 h after reperfusion in EA group was significantly smaller comparing with that in Is group (P<0.05). Conclusion：Electro-acupuncture preconditioning could increase CBF velocity in artery and vein of focal cortex in rats with MCAO, which also had time-dependence. Additionally, electro-acupuncture preconditioning could decrease the infarct volume detected 24h after reperfusion, which possibly was correlated with the blood supply in ischemic penumbra.