有氧运动通过胃促生长素调节肥胖及肥胖抵抗大鼠摄食量的研究

关键词：高脂饮食  有氧游泳运动  肥胖  肥胖抵抗  胃促生长素

Keywords:high-fat diet  aerobic swimming exercise  obesity  obesity resistant  ghrelin

摘要：

      摘要 目的：探讨有氧运动对肥胖及肥胖抵抗大鼠摄食量的影响及机制。 方法：4周龄雄性Sprague-Dawley大鼠，随机分为普饲对照组（C组）和高脂饲料组，饲养10周后，筛选出高脂饮食诱导的肥胖组（OB组）大鼠和肥胖抵抗组（OR组）大鼠。再分别将OB组和OR组随机分为运动组（OB-Sw组，OR-Sw组）大鼠和非运动组（OB-Sed组，OR-Sed组）大鼠，运动组大鼠进行8周60%的个体力竭强度的有氧游泳运动，45min/d，5次/周，每周监测大鼠体重和摄食量。8周干预结束后，取血清和胃组织，血清检测TG、TC、LDL-c、HDL-c，胃组织检测促生长素（Ghrelin）表达情况。 结果：10周后，高脂饲料组大鼠体重与普饲对照组相比，显著性增高（P＜0.05）；OB-Sed组大鼠的血清低密度脂蛋白（LDL）与C组的相比，显著性增高（P＜0.05）；通过8周的有氧运动，OB-Sed组大鼠的相对摄食量多于OB-Sw组，OR-Sed组大鼠的相对摄食量多于OR-Sw组；OB-Sed组、OR-Sed组大鼠胃组织Ghrelin表达程度高于OB-Sw组、OR-Sw组和C组。 结论：运动可通过降低大鼠胃组织Ghrelin的表达来降低摄食量。

Abstract:

      Abstract Objective: To explore the effect of aerobic exercise on the food intake of obesity rats and obesity resistant rats and some mechanisms Method: The four years old Sprague-Dawley rats were randomly divided into standard chow group (C) and high-fat diet group. After 10 weeks feeding, the obese group (OB group) and obesity resistant group (OR group) induced by high-fat diet were screened out. Then all the rats in OB group and OR group were respectively and randomly divided into exercise rats (OB-Sw group, OR-Sw group) and non-exercise rats (OB-Sed group, OR-Sed group). Aerobic swimming exercise for the exercise rats was defined as 60% of individual exhaustion exercise intensity, 45 minutes per day, 5 times per week. Meanwhile, the body weight and food intake of the exercise and non-exercise rats were monitored every week. After 8 weeks intervention, the serum and gastric tissue of rats were obtained, and then the TG, TC, LDL-c, HDL-c in serum and the expression of ghrelin in gastric tissue were detected. Result: Ten weeks later, the body weight of the rats with high-fat diet showed a significant difference compared with the rats with standard chow group (P＜0.05). The content of LDL in OB-Sed group was significantly higher than that in C group (P＜0.05). After 8 weeks aerobic exercise, for the food intake, OB-Sed group was higher than OB-Sw group and OR-Sed group was higher than OB-Sw group. The expression of ghrelin in gastric tissue in OB-Sed group and OR-Sed group was higher than that in OB-Sw group, OB-Sw group and C group. Conclusion: Exercise might reduce the food intake of obesity rats and obesity resistant rats by reducing the expression of ghrelin in stomach.