不同强度运动调控AMPK-SIRT 3通路对自发性高血压大鼠血管功能的影响

关键词：运动强度  AMPK-SIRT 3  自发性高血压大鼠  血管功能

Keywords:exercise intensity  AMPK-SIRT 3  spontaneously hypertensive rats  vascular function

摘要：

      摘要 目的：研究不同强度运动对自发性高血压大鼠(spontaneous hypertensive rats，SHR)主动脉血管功能的影响及其可能的机制。 方法：12只京都Wistar大鼠（Wistar-Kyoto Rats，WKY）为安静对照组(WKY-C，n=12)，48只SHR随机分为安静对照组(SHR-S，n=12)，低强度运动组(SHR-L，n=12)，中强度运动组(SHR-M，n=12)和高强度运动组(SHR-H，n=12)。运动组进行14周，每周5次，每次60mins的跑台运动训练。14周运动训练结束后取出胸主动脉，进行血管反应性实验检测主动脉血管功能，Western Blot检测主动脉蛋白水平表达量。 结果：SHR-S组与WKY-C组比较血管功能受损，内皮型一氧化氮合成酶(eNOS)、磷酸化一氧化氮合成酶(p-eNOS)表达下调，血管组织氧化应激水平升高，AMPK-SIRT 3表达下调。运动训练后，SHR-L组和SHR-M组血管功能与血管组织氧化应激水平相比较SHR-S组有明显改善(P<0.05)。SHR-H组与SHR-S组比较血管功能与血管组织氧化应激水平没有改善，乙酰胆碱依赖的血管舒张功能与内皮型一氧化氮合酶的功能降低。与SHR-S组比较，SHR-L组和SHR-M组AMPK-SIRT 3表达水平明显上调(P<0.05)，而SHR-H与SHR-S组比较没有明显改变。 结论：低、中强度运动可以有效减轻SHR的主动脉氧化应激并改善血管功能，而高强度运动不能改善SHR的血管功能，其机制可能与AMPK-SIRT 3通路有关。

Abstract:

      Abstract Objective： To study the effects of exercise training with different intensities on the aortic vascular function of spontaneously hypertensive rats (SHR) and the possible mechanism. Method： Twelve Wistar-Kyoto Rats（WKY）served as the quiet control group (WKY-C， n=12)， 48 SHRs were randomly divided into quiet control group (SHR-S， n=12)， low-intensity exercise group (SHR-L， n=12)， the medium-intensity exercise group (SHR-M， n=12) and the high-intensity exercise group (SHR-H， n=12). The rats in the exercise group were trained on treadmill with different intensities for 14 weeks， 5 times per week for 60 mins. After training， the thoracic aorta was taken out for testing. Vascular reactivity test was used to measure aortic vascular function， and Western Blot was used to detect aortic protein expression. Result： Compared with WKY-C， the vascular function of SHR-S was impaired， the expression of endothelial nitric oxide synthase(eNOS) and phosphorylated nitric oxide synthase(p-eNOS) were down-regulated， the oxidative stress levels was increased， and the AMPK-SIRT 3 pathway was inhibited. After exercise training， the vascular function and oxidative stress level of SHR-L and SHR-M significantly improved compared with SHR-S (P<0.05). However，the vascular function and oxidative stress level did not get improved in SHR-H compared with in SHR-S. In addition， the AMPK-SIRT 3 pathway in SHR-L and SHR-M were significantly activated compared with in SHR-S (P<0.05)， whereas there was no significant change in SHR-H. Conclusion： Low-intensity exercise and moderate-intensity exercise can effectively reduce the oxidative stress of the aorta of SHR and improve the vascular function， while high-intensity exercise cannot improve the vascular function of SHR. The mechanism may be related to the AMPK-SIRT 3 pathway.