分米波促进周围神经急性损伤康复的作用机制

关键词：分米波  周围神经损伤  雪旺细胞  康复

Keywords:decimeter wave  peripheral nerve injury  Schwann cell  rehabilitation

摘要：

      目的：探讨分米波促进周围神经急性损伤康复作用的机制。方法：选取体重200—250g健康SD大鼠30只，随机分成A（分米波治疗组）、B（空白对照组）两组。于右侧大腿制备坐骨神经急性损伤模型。A组于术后第1天至术后8周，损伤局部行分米波辐射，B组行空白对照。分别于术后不同时相对损伤神经进行大体解剖、光镜和电镜观察、轴突图像分析、免疫组织化学检测。结果:形态学观察术后不同时相内A组损伤神经恢复情况优于B组，免疫组织化学染色显示术后不同时相A组雪旺细胞中S-100蛋白表达水平高于B组，轴突图像分析A组有髓神经纤维数目、直径及髓鞘厚度均大于B组（P＜0.01)。结论:分米波能通过抑制炎性反应、减轻损伤后神经的充血水肿及与周围组织的粘连、促进雪旺细胞增殖等机制促进周围神经急性损伤后的康复。

Abstract:

      Objective: To approach the mechanism of the effect of decimeter wave irradiation on the repair of acute-injured nerves. Method: Thirty Sprague-Dawley(SD) rats(200—250g) were randomly divided into 2 groups: group A, the decimeter wave treated group and group B, the blank control group. The right sciatic nerves were crushed with forceps to form the experimental animal model of peripheral acute-injured nerves. Then the rats of group A were fixed on a table prostrated and the right posterior thighs were exposed to decimeter wave from the1st day to 8th week postoperatively. The rats of group B were also fixed on a table prostrated at the same time, but not exposed to decimeter wave. At different time points postoperation the samples were observed by anatomical exam, light and electron microscope observation, morphometric analysis and S-100 protein immunochemical staining. Result: The morphology observation showed that at different time points the recovery of nerves in group A were better than that in group B. The immunohistochemical staining showed S-100 protein expression in Schwann cells of the injured nerves in group A were more than that in group B. The morphometric analysis showed that in group A there were more myelinated axons counts, large axon mean diameters and myelin sheath mean thickness compared with those in group B(P<0.01). Conclusion:The mechanism of decimeter wave irradiation on the repair of acute-injured peripheral nerves was that decimeterwave could restrain the inflammation, alleviate the congestion and relieve the adhesion of the acute-injured nerves and promote the proliferation of Schwann cells.