电针对脑缺血再灌注大鼠学习记忆能力及MMP-2、MMP-9蛋白表达的影响

关键词：脑缺血  电针  学习记忆  基质金属蛋白酶-2  基质金属蛋白酶-9

Keywords:cerebral ischemia  electroacupuncture  learning and memory  matrix metalloproteinase-2  matrix metalloproteinase-9

摘要：

      摘要 目的：探讨电针神庭、百会穴对局灶性脑缺血大鼠学习记忆的影响，及其可能的机制。 方法：雄性SD大鼠45只，随机分为假手术组、模型组、电针组，每组15只大鼠。模型组、电针组均采用线栓法制备局灶性脑缺血损伤大鼠模型。电针组电针神庭、百会穴7d。各组在造模后第3天开始采用Morris水迷宫检测学习记忆能力；2,3,5-氯化三苯基四氮唑染色法检测大鼠脑梗死的体积；Western blotting法检测大鼠左侧海马基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9(MMP-9)蛋白的表达。 结果：同模型组相比，电针组大鼠学习记忆能力改善(P<0.05)，脑梗死体积明显减少(P<0.01)，海马组织中MMP-2、MMP-9蛋白表达降低(P<0.05)。 结论：电针能改善局灶性脑缺血再灌注大鼠学习记忆能力，其机制可能与抑制MMP-2、MMP-9蛋白表达相关。

Abstract:

      Abstract Objective：To explore the effect of electroacupuncture at Shenting (BL24) and Baihui (DU20) on learning and memory abilities in rats after cerebral ischemia/reperfusion (I/R) injury and its possible mechanism. Method：Forty-five male SD rats were randomly divided into sham group (n=15), ischemia group (n=15) and electroacupuncture group (n=15). The latter 2 groups were modeled as focal cerebral ischemia/reperfusion (I/R) injury, and electroacupuncture group received electroacupuncture at Shenting (BL24) and Baihui (DU20) for 7 days. Learning and memory abilities were tested by Morris water maze, cerebral infarction volume was measured by TTC staining and image analysis, and protein expressions of MMP-2 and MMP-9 were detected with Western blotting. Result：Compared with ischemia group, learning and memory abilities improved (P<0.05), cerebral infarction volume reduced (P<0.01), and expressions of MMP-2 and MMP-9 in hippocampus decreased (P<0.05) in electroacupuncture group. Conclusion：Electroacupuncture can improve learning and memory abilities from cerebral ischemia/reperfusion injury in rats, which may be related with the inhibition of expressions of MMP-2 and MMP-9 in hippocampus.